Extended Data Fig. 9: Strictly hepatotropic viral infection induces iHALT with oligoclonal expansion and comparable levels of SHM compared to the spleen during systemic infection.
From: iHALT unlocks liver functionality as a surrogate secondary lymphoid organ
(A-D) Proportion of IGHJ gene family usage in n = 1 naïve spleen, n = 3 LCMV-infected spleens (week 4 post-infection), n = 3 RHV-infected livers (week 4 post-infection), and n = 1 naïve liver with (A-D) two-tailed, unpaired t-tests. (E) Chord diagrams representing IgH V-J gene family pairing. Representative clonal lineage trees displaying nucleotide divergence from germline clones obtained from (F) naïve spleen, (G) naïve liver, (H) RHV-infected liver, and (I) LCMV-infected spleen. (A-D) Mean + SEM. (A-D) Two-tailed, unpaired t-tests were performed for LCMV spleen vs. RHV liver. For (A-D) LCMV spleen vs. RHV liver: (A) p = 0.0039, (B) p = 0.0099, (C) p = 0.2334, (D) p = 0.0780. Statistical significance was denoted as **=(p≤0.01).
