Fig. 5: Predictive potential of NeuMAP.
a, K-mass score of neutrophils from mouse lung cancer with or without with anti-CD40 immunotherapy27, mapped onto NeuMAP. b, Signature scores from human neutrophils isolated from blood of healthy individuals or patients with severe COVID-19 (ref. 31, influenza A28 or systemic lupus29, or from lungs of patients with cancer12. c, Representative images from spatial transcriptomics of lung sections from patients with lung adenocarcinoma, with healthy tissue, adjacent tissue and tumour lesion from the same individual. Top, H&E-stained sections. Middle, spatial distribution of neutrophil gene signature scores. Bottom, enlarged views of indicated regions showing neutrophils from different clusters or hubs. Data are from n = 8 patients, with 2 formalin-fixed paraffin-embedded (FFPE) tumour sections and 1 adjacent non-tumorous section per patient. Representative images are shown. Scale bar: 200 µm (main image); 50 µm (expanded view). d, Left, UMAP embedding of neutrophil transcriptomes from the spatial transcriptomic dataset across all regions, identifying transcriptional clusters shown in c. Middle, bar plot showing relative abundance of each neutrophil state in healthy, adjacent and tumour areas. Right, heat map showing mean score per hub for each human cluster gene set (scaled by signature). Kruskal–Wallis test,P < 0.001 for all hubs. e, Percentage of nearest neighbouring cells to neutrophils from different clusters or hubs. Numbers indicate cells scored per group. AT2, alveolar type 2 cell; TAM, tumour-associated macrophage. f, Overview of 18 physiological and pathological conditions from which single-cell blood neutrophil transcriptomes were obtained. 80 w.o., 80 weeks old. g, Top, UMAP analysis of blood neutrophils from f, coloured by sample origin. Overlap across samples and NeuMap hubs (defined in Fig. 1) measured by Bhattacharyya index. Bottom, projection of blood neutrophil transcriptomes onto reference NeuMAP embedding; overlap is quantified in a correlation matrix. h, Bhattacharyya indices showing overlap of each sample with NeuMap regions (Extended Data Fig. 10C), represented as a barcode for each condition in a hierarchical tree. Drawings in a–c,f were created in BioRender. Cerezo Wallis, D. (2025) https://BioRender.com/pfm336w.
