Extended Data Fig. 2: Identification of clusters and transcriptional hubs in the NeuMap.
(A) Score expression of gene sets from specific biological processes. See Supplementary Table 2 for a complete gene list. (B) Clustering of the NeuMap with different levels of resolution. The clusters that best captured the functional areas were selected across the different resolutions to annotate the transcriptional hubs of the NeuMAP (as in Fig. 1). (C) Clustering tree of 151,960 neutrophils from R0.05 to R0.3, with clusters labeled according to their size. The arrow intensity reflects the proportion of cells and the color of the number of cells assigned to the other clusters. (D) Heatmap of the differentially expressed genes for each transcriptional hub. For a complete gene list see Supplementary Table 3. (E) Clustering of the NeuMap at resolution 0.2. (F) Transcriptional hubs from (B) and mRNA expression of the indicated genes. (G) Heatmap showing the enrichment scores of the functional signatures in the transcriptional hubs (H) Heatmap showing the proportion of cells in all the conditions included in the NeuMap that contribute to the top 5% expressing cells for each indicated functional gene signature. The number of cells per tissue was downsampled to 1000 prior to the calculation of the contribution proportion.
