Extended Data Fig. 3: Architecture of the NeuMap across sex, environmental conditions, mouse strains, and genetic alterations.
(A) Experimental strategy and projection onto the NeuMap to assess neutrophil distribution across transcriptional hubs. (B) K-mass density plots and stacked bar graphs showing hub distribution of neutrophils from healthy tissues and influenza-infected lungs, separated by sex (male and female). (C) K-mass scores and hub distributions of neutrophils isolated from indicated tissues in naïve male mice housed under germ-free (GF), specific pathogen-free (SPF), or rewilded (RW) environmental conditions. (D) K-mass scores and hub distributions of neutrophils from healthy tissues, flu-infected lungs, and tumors in C57BL/6 J and BALB/c mice, showing conserved hub structure across mouse strains. (E) K-mass scores and hub distributions of wild-type and Tet2−/− neutrophils isolated from non-irradiated bone marrow chimeras, under control (upper panels) or hypercholesterolemic (lower panels) conditions, illustrating hub preservation across genetic backgrounds. WD, Western diet.
