Extended Data Fig. 4: Architecture of the human neutrophil compartment (human NeuMap).
(A) UMAP visualization of human neutrophils colored by tissue of origin. Data were downsampled to 3,000 cells per cluster for visualization. A complete list of samples is provided in Supplementary Table 4. (B) Two-dimensional kernel density estimation showing cell density distributions across tissues. (C) Module scores for selected biological processes (left) and for human neutrophil signatures recently described in ref. 18 across 12 cancer types (right). Gene lists are provided in Supplementary Table 2. (D) Functional compartmentalization of the human NeuMap. Each hub is defined as the area containing the top 85% K-mass score. For a complete gene list of differentially expressed genes for each transcriptional hub see Supplementary Table 4. (E) Stacked bar plots showing the proportion of neutrophils across different organs and conditions within each hub, defined in (D). (F) Score values of murine hubs across the human NeuMap, showing preferential distributions matching the hubs defined in the human NeuMap. (G) Gene Module scores of murine hubs projected onto human neutrophil hubs, scaled by row. CRC, colorectal cancer; BM, bone marrow; SLE, systemic lupus erythematosus. Drawings in b,g were created in BioRender. Cerezo Wallis, D. (2025) https://BioRender.com/pfm336w.
