Extended Data Fig. 5: Altered cortical size, laminar organization, neuronal morphology, neuronal projections, and connectivity in MADM labeled Tsc1/2 Emx-cKO cortex.
From: TSC tunes progenitor balance and upper-layer neuron generation in neocortex
(A,B) Expansion of cortical size in MADM labeled Tsc1/2 Emx-cKO brains. (C,D) TSC1/2 deletion leads to disrupted laminar organization and neuronal misplacement (circle; D) in MADM labeled Tsc1/2 Emx-cKO brains. (E,F) Changes in the morphology and increased branching of MADM labeled upper layer neurons (compare areas indicated by asterisk [E, F]). (G) Quantification of increased neurite branching in Tsc1/2 Emx-cKO cortex (n = 12 mice/group). (H,I) Consistent with the expanded upper layer neurons, the thickness of corpus callosum (CC) containing axons of upper layer neurons traversing to the contralateral cortex is increased in Tsc1/2 Emx-cKO. Bar (H, I) indicates callosal width. (J) Quantification of increased callosal width in Tsc1/2 Emx-cKO. Data shown are mean ± SEM (n = 5 mice/group). Unpaired two-sided t-test; **p < 0.01 and ***p < 0.001 [p = 0.0002 (G), 0.0022 (J)]. Scale bar: A-B (200 μm); C-D (50 μm); E-F (20 µm); H-I (100 µm).
