Extended Data Fig. 3: Effect of TSC1/2 deletion on pS6 expression.
From: TSC tunes progenitor balance and upper-layer neuron generation in neocortex
(A,B) Increased pS6 expression in TSC1/2 cKO cortex. (C) Quantification of changes in pS6 expression in TSC1/2 cKO cortex (n = 6 mice/group). (D) Immunoblot analysis and quantification (E) of increased pS6 expression in TSC1/2 cKO cortex (n = 4 mice/group). (F,G) Increased pS6 expression in TSC1/2 cKO RGCs (arrowhead). (H) Quantification of changes in pS6 expression in TSC1/2 cKO RGC soma (n = 25 soma). RGCs were co-labeled with RC2 antibodies. CP, cortical plate; IZ, intermediate zone; VZ, ventricular zone. Data shown are mean ± SEM. Unpaired two-sided t-test; ***p < 0.001 [p = <0.0001 (C), 0.0001 (E), <0.0001 (H)]. Scale bar: A-B (100 μm); F-G (50 µm). Sample RGC soma are outlined (dotted lines; F-G). A.U., arbitrary unit of pS6 intensity normalized to background. For immunoblot source data, see Supplementary Fig. 1.
