Extended Data Fig. 1: Power analysis for detecting a diverse clonal architecture in 1024 LUAD.

a, The scatter plot illustrates the relationship between the number of reads per chromosome copy (NRPCC) and the total detected single nucleotide variants (SNVs). Our ability to detect subclones relies not on the number of identified SNVs, but on the number of reads per tumour chromosomal copy. NRPCC accounts for tumour purity, ploidy, and sequencing coverage. b, The minimum cancer cell fraction (CCF) of the detected clusters in each tumour is plotted against NRPCC. To mitigate biases, we exclusively considered tumours with NRPCC ≥ 10. In these tumours, our analysis is sufficiently powered to identify a subclone with a CCF ≥ 30%. The suggested NRPCC threshold is denoted by the dashed line.