Fig. 4: Mechanism of tRNA binding and cleavage by Ba1Cas12a3.
From: RNA-triggered Cas12a3 cleaves tRNA tails to execute bacterial immunity
a, Cryo-EM structure of the Ba1Cas12a3–crRNA binary complex. b, Cryo-EM structure of Ba1Cas12a3–crRNA–target RNA ternary complex. c. Cryo-EM structure of the Ba1Cas12a3–crRNA–target RNA–tRNAAla(UGC) quaternary complex. d, Cryo-EM structure of the Ba1Cas12a3–crRNA–target RNA–tRNA tail quaternary complex in the post-cleaved state. e, Ba1Cas12a3 domain motion trajectories from the binary to the ternary complex. f, Ba1Cas12a3 domain motion trajectories from the ternary to the quaternary complex. g, Ba1Cas12a3 domain motion trajectories followed by release of the cleaved tRNA.
