Extended Data Fig. 1: Predicted domain organization of MpCas12a2, Ba1Cas12a3, and ApCas12a4, together with an approximate maximum-likelihood phylogeny of Cas12a (dark blue), Cas12a2 (red), Cas12a3 (purple), and Cas12a4 (yellow) amino acid sequences as well as amino acid sequence alignment of Cas12a2, Cas12a3, and Cas12a4.
From: RNA-triggered Cas12a3 cleaves tRNA tails to execute bacterial immunity
The phylogenetic tree was constructed using IQ-TREE with ultrafast bootstrapping (1,000 replicates). The scale bar at the bottom indicates the expected number of substitutions per site. The corresponding simplified phylogeny is shown in Fig. 1a. The alignment highlights key motifs and residues, including those involved in protospacer-flanking sequence (PFS) recognition, the aromatic clamp regions interacting with cleaved and non-cleaved strands, CRISPR RNA (crRNA) recognition, RuvC domain motifs, and zinc-ribbon sites. A subset of the amino acid alignment is shown in Fig. 1b.
