Extended Data Fig. 4: Assessing plasmid interference in E. coli in the absence of the stringent response regulator relA.
From: RNA-triggered Cas12a3 cleaves tRNA tails to execute bacterial immunity
(a) Confirmation of deletion of relA in E. coli BL21(AI). Top: two-step approach to generate the complete deletion of relA using lambda-RED recombineering. Primer pairs to verify the deletion are shown at each step. Bottom: Resolved PCR products at each step of generating the ∆relA strain. For gel source data, see Supplementary Fig. 1. (b) Transformation fold-reduction as part of the plasmid interference assay using the ∆relA strain. See Fig. 1d for an overview of the assay. Each dot represents a biological replicate starting from a separate colony. Bars and error bars represent the mean ± standard deviation of biological replicates (n = 3 or 4). Kan, kanamycin; Cm, chloramphenicol.
