Extended Data Fig. 5: Cryo-EM data processing of the MARV GPΔMucWT-MARV16 Fab complex.
From: Potent neutralization of Marburg virus by a vaccine-elicited antibody
a,b, Representative cryo-EM micrograph (a) and 2D class averages (b) obtained for MARV GPΔMucWT in complex with MARV16 Fabs. Scale bar: 100 nm. c, Cryo-EM data processing workflow. NUR (per-particle defocus): non-uniform refinement with per-particle defocus refinement. d, Gold-standard fourier shell correlation curves for the locally refined MARV GPΔMucWT-MARV16 Fab complex (using a mask comprising the GP trimer and MARV16 Fab variable domains). e, Local resolution calculated with cryoSPARC for the locally refined MARV GPΔMucWT-MARV16 Fab complex. f, Heat map of angular distribution for the particles contributing to the final reconstruction. g, Conical fourier shell correlation plot86.
