Extended Data Fig. 2: Analysis of binding and neutralization titres for the monoclonal antibodies discovered from ATX-Gx mice.

a, Flow cytometry gating strategy used for sorting MARV GPΔMuc_WT-reactive memory B cells. b,c, Dose-response curves for the ELISAs against the MARV GPΔMuc_WT ectodomain (b) and neutralization assays against VSV pseudotyped with MARV/Musoke GP (c) for the 10 antibodies discovered from the immunization study using the ATX-Gx mice. Two biological replicates were performed for the ELISAs using distinct batches of proteins and antibodies. Two technical replicates were performed per biological replicate. Data presented are from one representative biological replicate and presented as mean ± standard error from the two technical replicates. Two to six biological replicates were performed for the neutralization assays using distinct batches of antibodies and pseudoviruses. Three technical replicates were performed per biological replicate. Data from all biological replicates are shown and presented as mean ± standard error from the three technical replicates. d, Dose-response curves for plaque reduction neutralization tests for MARV16, MR78, MR191, and the MERS-CoV 4C2 IgG, conducted using authentic MARV/Musoke. Two biological replicates were performed with one to three technical replicates using distinct batches of monoclonal antibodies. Data are shown as the mean ± standard error of the technical replicates.