Fig. 1: Fasting induces H3K27ac changes in TMX-treated xenografts.

a, Schematic representation of the treatment cycles of mice xenografted with MCF7 cells. After tumours reached a palpable size, mice were treated with the different treatment arms. After four weeks of treatment, tumours were collected and the represented multiomics profiling was performed. TF, transcription factor. Adapted from Servier Medical Art (https://smart.servier.com), CC BY 4.0. b, Xenograft tumour growth in six-to-eight-week-old female athymic nude mice randomized in control arm (ad libitum diet, n = 6) or fasting alone (48 h weekly, n = 6), TMX alone (n = 6) or TMX combined with fasting (n = 6) treatment arms. n represents number of tumours per treatment group. Data are mean ± s.e.m. P values by mixed-effect model with Tukey’s multiple test correction (Supplementary Information) and two-tailed Student’s t-test (P values from the last day are represented). c, Heat map depicting the differentially enriched H3K27ac regions between TMX alone (n = 3) or TMX plus fasting (n = 6). n represents number of tumours per treatment group. Colour scale represents the average normalized read counts. d, Representative snapshots of differentially enriched regions for H3K27ac between TMX (blue) and TMX plus fasting (green). The genomic coordinates are annotated. e, Heat map of differentially enriched H3K27ac signal in xenografts treated with TMX or TMX plus fasting. f, In silico GIGGLE analysis for factor enrichment at TMX-enriched H3K27ac sites (top left, n = 3) or TMX plus fasting-enriched H3K27ac sites (bottom left, n = 6). n represents number of tumours per treatment group. Right, average binding motifs of the depicted transcription factors, using HOMER software. In box plots, boxes indicate the first and third quartiles, the centre line indicates the median, and the whiskers indicate the first and third quartiles expanded by 1.5× the interquartile range (Supplementary Information).

Source data