Extended Data Fig. 2: GR and PR chromatin binding is induced by fasting treatment.
From: Fasting boosts breast cancer therapy efficacy via glucocorticoid activation
a. PCA plots for GR, PR and c-JUN ChIP-seq regions between the 4 conditions in MCF7 xenografts. b. Genomic distribution of GR, PR and c-JUN ChIP-seq regions between the 4 conditions. c. Heat map of GR and PR ChIP-seq signal for all 4 conditions, at differentially-enriched H3K27ac sites between TMX and TMX+Fasting (top). Regions were sorted on H3K27ac signal. Data are centred at H3K27ac peak, within a ± 2 kb window. Average density plots is shown for ChIP-seq signal of GR and PR in xenografts for all 4 treatment conditions (bottom). Data are presented as mean values ± SEM. d. Heat map depicting ChIP-seq signal for c-JUN in MCF7 xenografts for all 4 treatment conditions, visualizing differentially-enriched c-JUN sites, comparing TMX and TMX+Fasting regions (top). Regions were sorted according to decreasing c-JUN signal. Data are centred at c-JUN peak, within a ± 2 kb window. Average density plots is shown for c-JUN ChIP-seq signal for all 4 conditions in TMX+Fasting enriched regions (bottom). Data are presented as mean values ± SEM.
