Extended Data Fig. 7: Competitive Schild analysis of fentanyl-stimulated GTPĪ³S binding in the presence of increasing concentrations of muzepan1 in mouse spinal cord.
From: GTP release-selective agonists prolong opioid analgesic efficacy
Data are normalized to 10āĀµM point of fentanyl as 100% and to vehicle alone (0%). The potencies of fentanyl are shown in the table inset. Muzepan1 alone is a full agonist, therefore, it stimulates binding at 10 and 100ānM when given alone (left x axis). Values for pEC50 and Emax are presented with 95% CI from analysis of the curves using GraphPad Prism (v. 10.4), (nā=ā7 individual mice with all three treatment groups assayed in parallel).
