Fig. 4: Potency in mouse hot plate and warm water tail immersion nociceptive assays.
From: GTP release-selective agonists prolong opioid analgesic efficacy
a–c, Morphine (a), Muzepan1 (b) and Muzepan2 (c) were tested at the indicated doses (in mg kg−1, intraperitoneal injection (IP)) in the hot plate (left) and tail flick (middle) assays in wild-type and MOR-knockout (MOR-KO) mice. Right, potency was determined by comparing the response at 1 h based on the percentage of MPE calculated from the baseline (BL) and a cut-off time of 20 s for the hot plate and 30 s for the tail flick. Potency curves are extended to convey 0% (determined by baseline) and 100% (determined by maximum cut-off time used to estimate the ED50); potencies are presented with 95% confidence interval in the legends. a, Morphine: n = 6 (3 mg kg−1 and 24 mg kg−1), n = 10 (6 mg kg−1 and 12 mg kg−1). b, Muzepan1: n = 8 (3 mg kg−1), n = 5 (6 mg kg−1 and 12 mg kg−1), n = 7 (24 mg kg−1); MOR-KO: n = 5. c, Muzepan2: n = 4 (6 mg kg−1), n = 10 (12 mg kg−1), n = 6 (24 mg kg−1); MOR-KO: n = 3. HP, hot plate assay; TF, tail flick assay.
