Fig. 1: Survival analysis and circulating viral burden of AGMs challenged with LASV Togo.
From: Oral 4′-fluorouridine rescues nonhuman primates from advanced Lassa fever
a, Kaplan–Meier survival curves of AGMs (n = 5) challenged with LASV. Survival data from cynomolgus macaque historical controls (HC, n = 5) from previous studies are included for comparison. Statistical significance was tested using the Mantel–Cox log-rank test. b, Viral load as measured by reverse transcription with quantitative PCR (RT–qPCR) amplification of LASV vRNA copy numbers in whole blood sampled at the indicated time points. Data from historical control cynomolgus macaques are provided for comparison. c, Plaque titration of circulating infectious virus from plasma sampled at the indicated time points. Data from historical control cynomolgus macaques are provided for comparison. d, Viral load in tissues from AGMs as assessed by RT–qPCR. vRNA copy numbers were assessed by RT–qPCR for tissues collected at necropsy. Data from historical control cynomolgus macaques are provided for comparison. ALN, axial lymph node; ILN, inguinal lymph node; Liv, liver; Spl, spleen; Kid, kidney; Adr, adrenal gland; RUL, right upper lung; RML, right middle lung; RLL, right lower lung; LUL, left upper lung; LML, left middle lung; LLL, left lower lung; BrFr, brain frontal cortex; BrSt, brain stem; CSC, cervical spinal cord; MLN, mandibular lymph node; sMnSG, submandibular salivary gland; Ton, tonsil; Hrt, heart; MsLN, mesenteric lymph node; Duo, duodenum; Pan, pancreas; Ile, ileocecal junction; TrCo, transverse colon; UrBl, urinary bladder; Gon, gonads; Ut/Pro, uterus/prostate; NaMu, nasal mucosa; Conj, conjunctiva; Eye, eye tissue. In b–d, data for individual monkeys and time points were derived from the mean of two replicate assays on the same biological sample. Data for the cynomolgus macaque historical control cohort are shown as the geometric mean titre ± geometric s.d. The horizontal dotted line indicates the lower limit of quantification (LLOQ) for each assay (1,000 GE ml−1 or 1,000 GE per g tissue for RT–qPCR, 25 PFU ml−1 for plaque titration).
