Extended Data Fig. 15: Optogenetic inhibition of ACC MOR+ cells induces a conditioned place preference in injured mice.

(a) Real-time place preference setup with Ethovision triggering an external LED system to administer blue light to the ACC of painTRAP2 mice transfected with the inhibitory chloride channel opsin, AAV-mMORp-DIO-iC + +. (b) Bilateral fiber optic design for LED light penetrance to the ACC (left), with a representative image of the cannula tracks over the dorsal Cg1 ACC expressing iC + + in painTRAP neurons. (c) Map of optic fiber placements across mice. (d) Design for optogenetic inhibition of ACC chronic nociceptive-MOR+ cell-types in a real-time place preference assay. (e) Cross-day changes in real-time, within-session self-administered inhibition via volitional selection to remain in an LED-triggered chamber, and subsequent conditioned place preference assessment with no LED exposure (n = 12 Uninjured mice, n = 14 SNI mice). (left Two-way ANOVA, Tukey correction: pVirus = 0.0066. right unpaired t-test: p = 0.0466). ⋆ = p < 0.05. Bars or dots are mean; lines and small dots/triangles are individual animals; error bars are SEM. See Table S1 Rows 156–157 for statistics.