Fig. 5: Post-intervention control of HIV is associated with a robust stem/memory-like, activated/proliferating CD8+ T cell response to rebound.
From: Correlates of HIV-1 control after combination immunotherapy
a,b, The gating strategy (a) and the frequency of Ki-67+ non-naive CD8+ T cells by manual gating at the baseline, pre-R and post-R1 timepoints as measured using CyTOF (b) (timepoint definitions are shown in Fig. 3). c, The distribution of Ki-67+ cells across CD8+ T cell subsets at the post-R1 timepoint. TN, naive (CD45RA+CCR7+CD95−); TSCM, stem cell memory (CD45RA+CCR7+CD95−); TCM, central memory (CD45RA−CCR7+), TTM, transitional memory (CD45RA−CCR7−CD27+), TEM, effector memory (CD45RA−CCR7−CD27−), TEMRA, CD45RA-expressing effector memory (CD45RA+CCR7−). d, The frequency of TCF-1+ cells within the Ki-67+ non-naive CD8+ T cells at the post-R1 timepoint. e, Uniform manifold approximation and projection (UMAP) analysis of non-naive CD8+ T cells including cells from all participants at the baseline, pre-ATI, pre-R and post-R1 timepoints (left). Right, expression of individual markers on the UMAP. f, The scaled median expression of markers across all clusters (activation markers CD38, HLA-DR and Ki-67 are boxed for emphasis). g, The frequencies of clusters from f of non-naive activated CD8+ T cells across the baseline, pre-R and post-R1 timepoints; c9, c12, c17, c8 and c14 are activated and express CD38 ± HLA-DR ± Ki-67; cells in c3 have a TEMRA phenotype. h, Two-sided Spearman correlations between each participant’s post-ART set point viral load and the frequency of select non-naive CD8+ T cell populations at the post-R1 timepoint. The analysis excludes PID 60610 (no rebound) and 35933 (no post-R1 sample). The dashed boxes in b, c and g encompass datapoints from post-intervention controllers.
