Extended Data Fig. 1: Overview of 166K-member gene circuit library construction.

a, Timeline of library construction process from level 0 part fragments to level 3 libraries. b, Schematic of the construction strategy for each level of the assembly process (grey boxes), with number of cells indicated for assembly transformation and plating (brown circles, 10 cm plates; brown rectangles, autoclave trays), colony scraping, and HEK-LP transfection (pink circles, 10 cm plates). The inputs and products for each assembly level are represented to the right. c, Nanopore sequencing read-length distribution of level 3 single-input inducible circuit library, with the relative proportion of each identifiable DNA product denoted: library members (pink), re-circularized level 3 destination vector (orange), empty level 3 destination vector (dark grey), contamination from level 0, 1, or 2 genetic parts (light grey), or unidentified DNA species (blue).