Extended Data Fig. 3: vCD44BP constrains effector but not memory CD8 T cell numbers.
From: Fibroblastic reticular cells direct the initiation of T cell responses via CD44
Representative flow cytometry plots showing (a) CD8 T cells, (b) IE1+ virus specific CD8 T cells and (c) IE1+ SLEC (CD127lo) and MPEC (CD127hi) subsets in spleens of BALB/c mice infected with MCMV or ∆vCD44BP (day 7 PI); plots are concatenated from one experiment (naïve n = 2; MCMV and ∆vCD44BP n = 4) and representative of three independent experiments. Representative flow cytometry plots showing (d) CD8 T cells and (e) IE1+ virus specific CD8 T cells in spleens of BALB/c mice infected with MCMV or ∆vCD44BP (day 40 PI); plots are concatenated from one experiment (naïve n = 2; MCMV and ∆vCD44BP n = 4) and representative of two independent experiments. Number of (f) CD8 T cells and (g) IE1+ virus specific CD8 T cells in spleens of BALB/c mice infected with MCMV or ∆vCD44BP at days 40 PI and 120 PI; pooled from two to four independent experiments (D40 PI: naïve n = 8; MCMV n = 8; ∆vCD44BP n = 10; D120 PI: naïve n = 8; MCMV n = 12; ∆vCD44BP n = 11). All mice were infected with 5 × 103 PFU. Mean is shown in flow plots; graphs show mean ± SEM; significance tested by two-sided Mann-Whitney.
