Extended Data Fig. 6: In vivo Perturb-seq with dual-guide RNA in LCMV chronic infection and individual validation.
From: Atlas-guided discovery of transcription factors for T cell programming
a, Retroviral vector design for dual-gRNA delivery. b, Feature plot of differentiation markers (Pan-exhaustion: red, TEXprog: pink, TEXeff: blue, Cell cycle: purple, TEXterm: brown). c, Heatmap of marker gene expression across cell state clusters identified by Seurat’s Find Markers() function. d, Frequency of PD1+SLAMF6−CD101+ cells (d). Frequency of CD38+CD39+ double-positive cells (e). Statistical analysis: Ordinary one-way ANOVA with Dunnett’s multiple comparisons test versus gScramble (n ≥ 5 from ≥2 biological replicates). Data are presented as mean ± s.e.m. ****P < 0.0001; ***P < 0.001; **P < 0.01; *P < 0.05.
