Extended Data Fig. 7: Single-cell transcriptomic profiling of CD8+ T cells from Perturb-seq in acute LCMV infection and validation of the selectivity of TRM single-state TF, Klf6 overexpression. | Nature

Extended Data Fig. 7: Single-cell transcriptomic profiling of CD8+ T cells from Perturb-seq in acute LCMV infection and validation of the selectivity of TRM single-state TF, Klf6 overexpression.

From: Atlas-guided discovery of transcription factors for T cell programming

Extended Data Fig. 7

a, Feature plots of differentiation marker genes (TCM: yellow, TEM: blue, TRM-Itgaelow: dark blue, TRM: green). b, Heatmap of differentially expressed genes between TRM-Itgaelow and TRM clusters. c, Volcano plots of differentially expressed genes in Cas9+ P14 CD8+ T cells expressing gEtv5, gArid3a, gHic1, or gGfi1. d, TRM single-state TF, Klf6 overexpression does not accelerate T cell exhaustion. Experimental setup: Klf6-RV or control-RV transduced P14 CD8+ T cells co-transferred into mice infected with chronic LCMV-Clone-13, Quantification of the frequency of PD1+, TEXprog (PD1+SLAMF6+CX3CR1−), TEXeff (PD1+SLAMF6−CX3CR1+) and TEXterm (PD1+SLAMF6−CX3CR1−) populations. Paired t-tests (n ≥ 6 from ≥2 biological replicates). Data are presented as mean ± s.e.m. ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05.

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