Extended Data Fig. 8: Depletion of TEXterm-single state TF, Zscan20 and TEXterm and TRM multi-state TF, Hic1 reduces T cell exhaustion.
From: Atlas-guided discovery of transcription factors for T cell programming
a, Experiment design. b, Representative flow plots of PD1+ P14 cells stained for SLAMF6 and TIM3. c, quantification of TEXprog (PD1+SLAMF6+TIM3−) and exhausted (PD1+SLAMF6−TIM3+) subsets. d, Frequency of PD1+CD39+ double-positive cells. e, Co-transfer of Cas9+ P14 CD8+ T cells transduced with RV-gZscan20 or RV-gHic1, mixed with gRNA control RV transduced cells into B16-GP33 tumor-bearing mice. f, Representative flow plots of PD1+ P14 CD8+ T cells stained for SLAMF6 and CX3CR1. g-i, Quantification of (g) TEXprog (PD1+SLAMF6+TIM3−), (h) TEXterm (PD1+SLAMF6−TIM3+), and (i) IFNγ+TNF+ population. Paired t-tests (n ≥ 6 from ≥2 biological replicates). Data are presented as mean ± s.e.m. ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05.
