Fig. 4: Heterochronic RG lineage progression under TF perturbations.
From: Dissecting gene regulatory networks governing human cortical cell fate
a, Stacked barplot showing distributions of clonal cluster fractions along human developmental stages in NT, NR2E1, ARX, SOX2, ZNF219 and NEUROD2 KD. Legend is shown in c. b–d, UMAPs of human clones coloured by pseudotime (b), fate biases (c) and individuals (d). e, Ridge plots of pseudotime distribution in clones grouped by stages. f, Boxplot showing median clonal pseudotime in each individual under TF perturbation, highlighting opposing roles of ARX and NR2E1 in regulating maturation of RG fate potential. Two-sided paired Wilcoxon tests; P = 0.0078 (NR2E1), 0.016 (ARX), 0.84 (SOX2), 0.0016 (ZNF219), 0.25 (NEUROD2). Centre line, median; box limits, upper and lower quartiles; whiskers, 1.5× interquartile range; points, outliers. g, Heatmap showing changes in gene expression in RG along clonal pseudotime. h, Violin plots showing gene expression changes in RG in ARX and NR2E1 KD. *P < 0.05; **P < 0.01; ***P < 0.001.
