Extended Data Fig. 5: A bacterial epitope display system for OVA antibody reactivity profiling.
From: Environmentally driven immune imprinting protects against allergy
(a) Creation of peptide epitope libraries for serological testing. Amino acid sequences of antigens were reverse translated, segmented into overlapping 36 bp tiles and cloned en masse into a display scaffold plasmid also coding for one of three fluorescent proteins. (b) Cartoon depiction of the eCPX scaffold on the bacterial surface, with displayed peptide in red. (c-e) The libraries were mixed with mAbs of known reactivities and screened by flow cytometry for binding (d) or protein G-enriched for sequencing (e). (f) Top 200 6mer epitopes enriched by OVA mAb1 from a universal epitope library. (g) Sequence logo of the top ten 6mer epitopes enriched by mAb1. Data are representative of two or more separate experiments.
