Extended Data Fig. 6: IL-4+ splenocytes as measured with ELISpot.
From: Environmentally driven immune imprinting protects against allergy
(a) Representative ELISpot images of IL-4+ splenocytes from the indicated sources incubated without antigen or with cOVA or KLH. (b-d) quantification of IL-4+ spots at baseline (b), after incubation with cOVA (c), or after incubation with KLH (d) (n = 8 SPF/null, 8 SPF/KLH, 9 pet shop/null). Statistical significance was determined using one-way ANOVA with Tukey correction for multiple comparisons. Error bars represent SD. Data are pooled from two or more repeats of each experiment.
