Fig. 2: Pet shop mice have pre-existing immune memory of model antigens.
From: Environmentally driven immune imprinting protects against allergy
a, cOVA-reactive IgG from sera of unimmunized SPF (C57BL/6J) or pet shop mice (SPF: n = 10; pet shop: n = 15) at 1:100 dilution. b, Epitope profiling of cOVA-reactive IgG in SPF (C57BL/6J) and pet shop mice with or without cOVA exposure (n = 6 baseline; 10 immunized). Each row represents one sample. FC, fold change. c, Representative ELISpot images of IFNγ+ splenocytes from the indicated sources incubated without antigen or with cOVA or KLH. d–f, Quantification of IFNγ+ spots at baseline (d) and after incubation with cOVA (e) or KLH (f) (SPF unimmunized: n = 8; SPF KLH: n = 8; pet shop unimmunized: n = 9). Colours as indicated in c. g, Representative flow cytometry plots of CD4+CD154+ T cells from the indicated sources following incubation with or without cOVA. h, Background-corrected quantification of IFNγ+CD4+ T cells after incubation with cOVA (SPF: n = 11; pet shop: n = 12). Two-tailed unpaired Student’s t-test (a,h) or one-way ANOVA with Tukey correction for multiple comparisons (d–f). Error bars represent s.d. Data are pooled from two or more repeats of each experiment or representative of two experiments (b).
