Fig. 5: Cross-tolerance protects mice from allergy.
From: Environmentally driven immune imprinting protects against allergy
a, Experimental scheme for the induction of oral tolerance by cOVA in water in SPF (BALB/cJ) mice. MQ water, Milli-Q water control. b, Measurement of core body temperature following systemic challenge in mice that were sensitized to and challenged intraperitoneally with cOVA. c,d, Maximal temperature decrease following systemic cOVA challenge (c) and cOVA-reactive serum IgE (d) (n = 20). Colour scheme as in a. e–j, Maximal temperature decrease (e,g,i) and antigen-reactive IgE (f,h,j) in mice sensitized to and challenged with the indicated OVA orthologue. Colour scheme as in a. e, n = 8. f, MQ: n = 11; cOVA: n = 15. g, MQ: n = 10; cOVA: n = 8. h, n = 10. i, MQ: n = 7; cOVA: n = 6. j, n = 8. k, Per cent protection of cOVA oral tolerance on sensitization to OVA orthologues. l, Experimental scheme for generating cohorts and measuring the effect of legume cross-tolerance. SPF (BALB/cJ) breeders were placed on chow or soy-free (and therefore, legume-free) diets. Progeny from each group were weaned onto matched diets and subcutaneously injected with the indicated legume extracts adsorbed to alum twice, with injections spaced one week apart. Serum samples were collected five days after the last injection, and mice were challenged intraperitoneally with matched legume extracts two days after serum collection. m, Maximal temperature decrease after systemic cOVA challenge (chow: n = 15; soy-free: n = 9). n–s, Maximal temperature decrease (n,p,r) and legume-reactive IgE (o,q,s) in mice sensitized to the indicated legume extract. n, Chow: n = 14; soy-free: n = 9. o, Chow: n = 17; soy-free: n = 9. p, Chow: n = 9; soy-free: n = 10. q, Chow: n = 15; soy=free: n = 11. r, Chow: n = 12; soy-free: n = 9. s, Chow: n = 12; soy-free: n = 8. Two-tailed unpaired Student’s t-test (c–j,m–s). Error bars represent s.d. (c–j,m–s) or s.e.m. (b). Data are pooled from two or more repeats of each experiment.
