Extended Data Fig. 2: Pet shop mice mount unusual antibody responses after sensitization.
From: Environmentally driven immune imprinting protects against allergy
(a-c) Comparison of antigen-reactive IgE (a), IgG1 (b) and IgG2 (c) following skin sensitization in the indicated inbred SPF strains and pet shop mice (n = 14 pet shop, 10 C3H/HeJ, 5 others). (d) Kinetics of antibody production by isotype following primary sensitization in SPF (BALB/cJ) and pet shop mice (n = 30 SPF, 31 pet shop). (e) Maximal temperature loss following systemic cOVA challenge in the indicated strains (n = 3 CAST/EiJ, 4 (BALB/cJ, C57BL/6 J, PERC/EiJ), 5 others). (f-g) Antigen-reactive antibody levels of the indicated isotype following lung (n = 24 SPF, 18 pet shop) (f) or intestinal (n = 21 SPF, 20 pet shop) (g) sensitization. Statistical significance was determined using one-way ANOVA with Tukey correction for multiple comparisons (a-c) or two-tailed unpaired Student’s t-test (e,f). Error bars represent SD. Data are pooled from two or more repeats of each experiment.
