Extended Data Fig. 8: Behavioural state prediction assessing the functional site of octopamine and tyramine production.
From: Predatory aggression evolved through adaptations to noradrenergic circuits
(a) Probability density map of velocity and pumping rate for genetic silencing of RIC and RIM neurons. Plots show tbh-1 mutant + 10 mM histamine and tbh-1; tdc-1p::HisCl without histamine controls and tbh-1; tdc-1p::HisCl + 10 mM histamine silencing conditions. (b) Relative time in each behavioural state for all conditions in (a). Box plots follow Tukey’s rule with the box from first to third quartiles, and a line at the median. The whiskers denote 1.5 x interquartile range. Statistics were performed with Mann-Whitney-U-test using Bonferroni correction for multiple tests against Ppa-tbh-1 +Histamine (black asterisks) and Ppa-tbh-1; Ppa-tdc-1::HisCl -Histamine (grey asterisks). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; n = 147 for Ppa-tbh-1 +Histamine, n = 79 for Ppa-tdc-1::HisCl -Histamine, n = 138 Ppa-tdc-1::HisCl +Histamine. (c) Average transition rates between behavioural states for tbh-1 + 10 mM Histamine, tdc-1p::HisCl without histamine and tdc-1p::HisCl + 10 mM histamine silencing conditions. The number in circles indicates the average state duration as in (b), and the arrow size indicates the transition rate normalized to outgoing transitions.
