Extended Data Fig. 12: Immunoprecipitation followed by mass spectrometry (IP-MS) of M5 regulators.
From: Developmental convergence and divergence in human stem cell models of autism
(a) Volcano plots show logFC of proteins compared to IGG controls. Highlighted are SFARI genes (1,2,S). Statistics were derived from 3 replicates using a two-tailed one-sample moderated t-test to calculate P values and FDR. (b) Significance of connectedness of networks is determined by comparing the network mean logFC to 10000 randomly selected networks of the same size across each of the protein baits. Red dotted line shows the network mean logFC and black line shows the 95th percentile. (c-d) Connectivity networks from gene lists of interest. Size of circle represents number of significant proteins for each bait. c) Full connectivity of significant interactions of all baits. d) NDD and ASD risk genes2,8,91.
