Fig. 2: Clustering and overlap of ASD forms points to increasing convergence across development. | Nature

Fig. 2: Clustering and overlap of ASD forms points to increasing convergence across development.

From: Developmental convergence and divergence in human stem cell models of autism

Fig. 2: Clustering and overlap of ASD forms points to increasing convergence across development.The alternative text for this image may have been generated using AI.

a, Heatmap of hierarchical clustering based on correlation of logFC between different conditions (ASD form and differentiation day). Annotation bars (shades of blue) represent differentiation day and colourful boxes represent ASD form. b, The number of DEGs in each ASD form during hCO differentiation. Colours and sizes of the circles represent the number of DEGs. c, Overlap in DEGs between forms of ASD at day 25. Number and percentage of total genes are indicated. Statistics were derived from Fisher’s exact test (two-sided). ***FDR < 0.005. d, Gene ontology terms significantly enriched in unique and intersecting DEGs. Statistics were derived from clusterProfiler enrichGO. e, Overlaps between DEGs at day 25 and ASD2,8, NDD and intellectual disability risk genes91. Colour represents OR and size represents −log10FDR. Only positive significant overlaps (OR > 1 and FDR < 0.05) are shown. Statistics were derived from Fisher’s exact test (two-sided). f, Network representation of the correlation (Spearman’s rho) between logFC of different ASD forms at days 25 and 100. Colour corresponds to rho value and line thickness corresponds to the rho absolute value. g, All correlations (Spearman’s rho) between logFC of the different forms. ANOVA followed by posthoc Tukey’s HSD; ANOVA F3,140 = 10.97, P = 1.6 × 10−6, Tukey HSD: day 100 to day 25 P = 2.67 × 10−5, day 100 to day 50 P = 8 × 10−6, day 100 to day 75 P = 7 × 10−4. Boxplots show: centre, median; lower hinge, 25% quantile; upper hinge, 75% quantile; lower whisker, smallest observation greater than or equal to lower hinge −1.5× interquartile range; upper whisker, largest observation less than or equal to upper hinge +1.5× interquartile range. Statistics were derived from n = 36 correlations per time point. ***Tukey HSD for all contrasts less than 0.001. h, The number of differentially expressed meta-significant genes comparing all affected forms with control participants. Colour and size of the circle represent the number of DEGs. BMP, bone morphogenetic protein; ID, intellectual disability.

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