Fig. 5: M5 regulators form a PPI network enriched for neurodevelopmental risk.
From: Developmental convergence and divergence in human stem cell models of autism
a, Western blots (run once per antibody) confirm antibody binding for proteins of interest. b, Example volcano plot shows significantly bound proteins. Highlighted proteins are SFARI risk genes. Statistics were derived from three replicates using a two-tailed one-sample moderated t-test with FDR correction and a total of 632 variables. NS, not significant. c, Z-score connectedness of gene lists show that M5 proteins detected in IP–MS experiments form a significant PPI and that this network is significantly connected with other neurodevelopmental risk such as SFARI genes (1, 2, S), NDD genes2,8,91 and genes enriched for developmental delay (DD), ASD or NDD broadly92. *FDR < 0.05, **FDR < 0.01, ***FDR < 0.005, z-score computed against a permutation-derived null; FDR-corrected P values obtained from standard normal approximation. d, Experimentally determined PPI network of M5 proteins tested in IP–MS experiements. e–f, Experimentally determined PPI networks of the five M5 baits used showing their interactions with SFARI genes (e) and risk genes as defined in Fu et al.92 (f). Size of circle represents the number of connections, edge width represents logFC between nodes from one direction of the IP for each UniProt accession number. Colour represents the gene list. Grey nodes are network baits not in the featured gene list.
