Extended Data Fig. 2: Dual effect of eGtACR1 at somatic and axonal compartments.
From: Holistic motor control of zebra finch song syllable sequences
a) Schematics (top), and in-vivo recordings under anhestesia (bottom) showing the dual effect of eGtACR1-mediated stimulation of axonal terminals causing neurotransmitter release (red), and somatic inhibition (blue). Sample traces, raster plots and PSTH (average ±SEM fold change in HVC activity binned each 100 ms, n = 5 hemispheres, 3 birds) reporting in-vivo recordings of HVC neuronal activity change in response to NIf axon terminal excitation (red) or soma inhibition (blue) upon light delivery (region shaded in green; scale bar 200 ms, 1 V). b) Sample traces of evoked EPSPs from an eGtACR1-expressing neuron in absence or presence of somatic light-mediated inhibition (green bar; current injection 100 ms 50 pA steps, −200 pA to +400 pA, scale bar 100 ms, 20 mV). Brain outlines adapted with permission from ref. 60, Wiley.
