Extended Data Fig. 6: Image processing procedure for the cryo-EM structure of STING bound to cGAMP/PI(3,5)P2/C53 and the quality of the structure.
From: Regulation of STING activation by phosphoinositide and cholesterol
(a) Image processing procedure. (b) Gold-standard FSC curve of the final 3D reconstruction (left) and FSC between the map and atomic model (right). (c) Local resolution map of the final 3D reconstruction. (d) Sample densities of various parts of the structure. (e) Detailed view of the binding modes of PI(3,5)P2 and CHS at the interface between two STING dimers in the structure of STING bound to cGAMP/PI(3,5)P2/C53. (f) Cryo-EM density of PI(3,5)P2 and CHS in the structure of STING bound to cGAMP/PI(3,5)P2/C53.
