Extended Data Fig. 8: Infectivity of An. stephensi MSQ43 cells to flaviviruses and the interaction between mosquito VCPs and flaviviral capsids were analysed, with the latter detected by SPR assay.
From: Mosquito–capsid interactions contribute to flavivirus vector specificity
a-c, The cells were infected with DENV2, ZIKV or JEV at an MOI of 0.01, respectively. Cell lysates were collected at 0, 24, 48, 72, and 96 h postinfection. Total RNA was extracted, and infectivity was detected by RT-qPCR. The viral load was normalized to that of Ae. aegypti actin (AAEL011197), Cx. quinquefasciatus actin (CPIJ012570) or An. stephensi actin (ASTEI20_033844). These data are presented as the mean ± s.e.m. Representatives from 2 independent experiments are shown. d-f, The binding affinity between mosquito VCPs and flaviviral capsids was measured by surface plasmon resonance (SPR). Mosquito VCPs and flaviviral capsids were expressed and purified in E. coli. The results are representative of 3 independent biological replicates.
