Extended Data Fig. 6: Comparison of NTSR1-Gi and NTSR1-G11 Complexes & Intermediate States.
From: Snapshots of the dynamic basis of NTSR1 G protein subtype promiscuity
a) Alignment of the Gα subunits of the Gi C-Open-Apo, Gi C-Closed*-GTP, G11 C-Closed-GTP, and G11 C-Closed*-GTP states, with insets highlighting the switch II and switch III regions of differing pairs. b) Alignment of the Gα subunits of the NTSR1-Gi1 C-Open-Apo and NTSR1-Gi1 C-Closed*-GTP states. c) Alignment of the Gi-C-Open-Apo, Gi-C-Closed*-GTP, and G11 C-Closed*-GTP states. d) Alignment of the Gi-C-Closed-GTP and G11-C-Closed-GTP states. e) Percent of NTSR1-G-protein contacts attributable to each region of NTSR1 in the C-Open-Apo and C-Closed-GTP states. f) Difference in percent of contacts from the G11-C-Open-Apo and G11-C-Closed-GTP states for NTSR1. g) Alignment of the sequences of the αN-β1 loop, β2-β3 loop, and α5 loop of Gi1 and G11. h) Alignment of the sequences of the αN-β1 loop, β2-β3 loop, and α5 loop of various Gi/o, Gq/11 family members, and Gs.
