Fig. 3: r.i. of the toxin + NTA formula prevents relapsing CDI.
From: Mucosal vaccination clears Clostridioides difficile colonization
a, Experimental schematic. The diagram was created using BioRender. b, Survival of mice 30 days after infection and relapse with WT C. difficile R20291. c, Weight loss. d, Stool score. e–j, Enumeration of total C. difficile R20291 bacteria (vegetative cells and spores; e,g,i;) and spores in faeces (f,h,j) after vaccination with dmLT only (e,f), toxins + dmLT (g,h) and toxins + NTAs + dmLT (i,j). P values were calculated relative to the control at the same timepoint. Individual lines correspond to individual mice. The limit of detection is shown by the dotted line: 500 CFU per g faeces. For c and d, data are mean ± s.e.m. Statistical significance was calculated using log-rank Mantel–Cox tests (b) and one-way ANOVA with Tukey’s correction (c and d, and e–j, with each day analysed individually). For b–j, n = 15 per group; decreases in n are due to animal mortality during infection.
