Fig. 4: r.i. of the toxin + NTA formula elicits robust anti-C40 peptidase 2 and anti-FlgGEK faecal IgG and a colonic TH17-associated tissue-resident memory cell response against CspC.
From: Mucosal vaccination clears Clostridioides difficile colonization
a–e, Sera IgG responses against antigens before challenge at day 28. f–j, Faecal IgA responses against antigens before challenge at day 28. k–o, Faecal IgG responses against antigens before challenge at day 28. p–s, Percentages of T cells in the IEL and LPL compartments of the colon at day 3 after infection. t–v, Enumeration of cytokines produced by ex vivo T cells from vaccinated mice that were co-cultured with CspC-primed dendritic cells in vitro. For a–v, data are mean ± s.e.m. Statistical significance was calculated using one-way ANOVA with Tukey’s correction (a–v). Individual datapoints are represented and were pooled from two independent experiments. For a–o, n = 5–15 per group (dependent on ability to collect samples); p–s, n = 4–10 per group (dependent on whether there was death at day 3 after infection); t–v, n = 3 replicates per group/condition. C40 pep 2, C40 peptidase 2; N, naive/no antigen mice.
