Extended Data Fig. 2: Pairwise sequence identity matrices for dCas12f, σE, HTH, and gRNAs.
From: Exapted CRISPR–Cas12f homologues drive RNA-guided transcription
a, Heatmap of pairwise amino acid sequence identity percentages among dCas12f homologues tested in this study. The matrix is color-coded by sequence identity (see legend inset), and percentages are listed. b, Pairwise amino acid sequence identity between tested σE homologues, shown as in a. The Bba locus lacks an rpoE gene. c, Pairwise amino acid sequence identity between tested HTH homologues, shown as in a. Note that Sda, Lby, and Pdi loci contain two hth genes, while this gene is absent in the Bba locus. d, Pairwise nucleotide sequence identity between tested gRNAs, shown as in a. e, Annotated genomic loci of all homologous dCas12f-σE systems tested in this study, with hth, rpoE, and dcas12f genes labeled and colored in yellow, blue, and red, respectively. Predicted functions for other neighbouring genes in grey are indicated by COG (Clusters of Orthologous Groups) letters, as indicated in the legend below.
