Extended Data Fig. 8: Features of the post-cleavage complex.
From: Mechanism of co-transcriptional cap snatching by influenza polymerase
a, Comparison of pre- and post-cleavage FluPol structures. Pre-cleavage is shown in turquoise, and post-cleavage is in pink. b, Cryo-EM density for the RNA within FluPol between the PB2 cap-binding and PA endonuclease domains. The density is colored by thresholds. Red corresponds 0.6 and purple to 0.3. The endonuclease active site Mg2+ is shown as a dark blue sphere. The density was restricted to areas close to the RNA in ChimeraX. The RNA is colored by heteroatom. c, Comparison of the Cryo-EM density of the RNA in the endonuclease for pre- and post-cleavage FluPol shows no density for the G11 nucleotide. The density was restricted to areas close to the RNA in ChimeraX using a wider cutoff. The density is shown in transparent gray and the RNA is colored by heteroatom. d, Schematic representation of the RNA and its interacting residues within the post-cleavage complex. The RNA is color coded by resolution using the same thresholds as in e, balck RNA residues are not modelled. e, The surface at the RNA exit of FluPol is positively charged, guiding the 3′-end of the cleaved RNA fragment into FluPol.
