Table 1 Phenotypic and genotypic characterization of recombinant viruses

From: Mechanism of co-transcriptional cap snatching by influenza polymerase

Virus

Titrea (PFU ml−1)

Plaque diameterb (mm)

Mutationc

Wild type

(5 ± 1.8) × 107

5.82 ± 0.68 (n = 51)

 

PA(Y131A)

(3 ± 0.33) × 103

2.92 ± 0.77 (n = 84)

PA(Y131A) (>99%)

PA(K104A/E141A)

(9 ± 0.62) × 107

4.37 ± 0.72 (n = 27)

PA(K104A) (>99%)

PA(E141A) (99%)

PB2(E452R)

(2 ± 0.37) × 107

4.55 ± 0.75 (n = 21)

PB2(E452R) (>99%)

PB2(S453P) (93%)

PB2(D466R)

(4 ± 0.28) × 107

5.72 ± 0.86 (n = 40)

PB2(D466R) (11%)

PB2(D466C) (79%)

PA(M211V) (27%)

PB2(K482E)

(1 ± 0.4) × 106

4.48 ± 0.66 (n = 30)

PB2(K482E) (99%)

M2(E75K) (12%)

  1. aDetermined using a plaque assay on MDCK cells (mean ± s.d. of technical triplicates). bDetermined using a plaque assay on MDCK cells (mean ± s.d. from 21 to 84 plaques, as indicated). cDetermined by Illumina sequencing after RT–PCR amplification of the whole viral genome. Only mutations that differ from the wild-type sequence and were found in more than 10% of the reads are indicated.
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