Fig. 1: Identification of the TC progenitor.
From: Ontogeny and transcriptional regulation of Thetis cells
a, Schema for BM and FL chimeras. b, Number of CD45.2+ TCs in FL and BM chimeras, as in a (n = 4 mice per group). c, Number of TC I–IV in mesenteric anlagen or mLNs at the indicated ages (n = 5 mice for E18.5 and n = 6 mice for all other time points). d, Frequency of tdTomato-labelled cDCs or TCs isolated from mLN of 2- or 9-week-old Il7rcreR26lsl-tdTomato mice (n = 4 per group). Each symbol represents an individual mouse. e, Schematic representation of the classical model of haematopoiesis. f, Schema for scRNA-seq on IL7R fate mapped and RORγt+ cells during FL and early postnatal haematopoiesis. g, Uniform manifold approximation and projection (UMAP) of 101,192 cells, as in f, coloured by cluster annotation. The TC cluster is circled in purple. NCR+ ILC3, natural cytotoxicity receptor-positive ILC3; Mono, monocyte; Mac, macrophage; Neu, neutrophil/granulocyte progenitor; Eos/Bas, eosinophil/basophil progenitor; Ery/Meg, erythroid/megakaryocyte progenitor; GMP, granulocyte-monocyte progenitor. h, UMAP embedding of 14,581 FL progenitors, TCs and ILCs subsetted from f and coloured by cluster annotation. i, UMAP with FL-derived cells coloured by unimputed expression of the indicated progenitor genes. Non-FL-derived cells are coloured in grey. j, UMAP coloured by TC fate probability or LTi fate probability. k, UMAP coloured by unimputed expression of Rorc (right). l, Subclustering of cells with high TC fate probability, shown in orange. m, Heatmap showing scaled, imputed expression of the top 20 differentially expressed genes (one versus the rest, fold change (FC) > 1.5, adjusted P < 0.01) for TLP, TCP, LTiP and TC I–IV clusters. n, Force-directed layout of cells as in h, coloured by cluster annotation. Arrows and lines represent the direction of expected cell-state transitions inferred from the CellRank transition matrix, showing progression or progenitor cells towards terminal states. o, UMAP coloured by unimputed expression of TLP signature genes. p, Flow-cytometry identification of TLP and expression of TLP markers by indicated progenitors in E18.5 FL from RorcVenus mice. Representative of n = 4 mice. Data in a–c are representative of 2–3 independent experiments; data in p are representative of more than 10 independent experiments. Each symbol represents an individual mouse. Error bars: mean ± s.e.m.; two-tailed unpaired t-test (b) or two-way analysis of variance (ANOVA; d).
