Extended Data Fig. 5: TLP are descended from IL7R⁺SiglecH⁺ lymphoid progenitors.

a, Violin plot of Il7r expression across clusters defined in Fig. 1h. b, UMAP of FL progenitors colored by unimputed expression of indicated genes. FL cells colored in grey. c, FACS-isolation of Lin(CD19, NK1.1, TCRβ, TCRγδ, Ter-119, Ly-6G, CD3e, FceR1a, CD90.2, CD88, Siglec-F)⁻FLT3⁺IL7R⁺CSF1R⁻Ly6D⁻CD27⁺ CLP, Lin⁻FLT3⁺SiglecH⁺ pDC/tDC progenitors, or Lin⁻FLT3⁺IL7R⁻CSF1R⁺MHCII^−/loCD11c^−/lo MDP/CDP progenitors from E18.5 Rorc^Venus liver. d, Frequency of APC subsets (left) among mLN Lin(CD19, TCRβ, Siglec-F^hi)⁻MHCII⁺ CD45.2⁺ cells, 6 days post transfer into CD45.1 P2 recipients (n = 6). Frequency of TC subsets (right) among CD45.2⁺ TCs in mLN 6 days post transfer into CD45.1 P2 recipients (n = 6). e, Competitive chimeras were generated with a 1:1 mix of CD45.2 Tcf4^STOP/STOP and CD45.1 wildtype E18.5 FL transplanted into lethally irradiated CD45.1 recipients and analyzed 4 weeks later (n = 5 recipients). Proportion of CD45.2 and CD45.1 cells among TC subsets in mLN, skin-draining peripheral lymph nodes (pLN) and spleen. Data in d pooled from five independent experiments, data in e representative of three independent experiments. Error bars: means ± s.e.m. One-way ANOVA (d). All P values are indicated on the corresponding graph.

Source data