Fig. 2: Establishing precursor–progeny relationships for TLP and TCP.
From: Ontogeny and transcriptional regulation of Thetis cells
a, UMAP of 1,138 Lin−CXCR6−RORγt+MHCII+ cells from the mLN of 2-week-old RorcVenus mice profiled by SS3. b, Heatmap reporting scaled, imputed expression of the top 20 differentially expressed genes for each cluster (one versus the rest, FC > 1.5, adjusted P < 0.01). c, Index sorting flow cytometry of cells in TC clusters identified in a. d, Flow plots for identification of TCP and TC I–IV subsets. Representative of n = 3 mice. e, TCPs and early TCs from the mLN of P11–12 RorcVenus mice were cultured ex vivo on mLN slices from 2–3-week-old CD45.1 mice. Representative flow cytometry of CD45.2+ progeny at 24 h and summary bar graph of cell numbers recovered for indicated cell types are shown (n = 4 mice). f, Expression of Siglecf in FL and TC progenitor clusters identified in Fig. 1h or mLN clusters in a; SS3. g, Expression of tdTomato in indicated cell types in mLN of P11 SiglecfcreR26lsl-tdTomatoRorcVenus mice (n = 8) and summary graph of frequency of tdTomato+ cells among the indicated cell types (NCR+ ILC3, natural cytotoxicity receptor+ ILC3). Each symbol represents an individual mouse. Data in d are representative of three independent experiments; data in e are pooled from two independent experiments; data in g are representative of two independent experiments. Each symbol represents an individual mouse. Error bars: mean ± s.e.m.; one-way ANOVA (e–g). All P values are indicated on the corresponding graphs.
