Extended Data Fig. 1: E. coli Nissle (EcN) colonization-induced systemic immunogenicity cross-reactive to neonatal infection clinical isolates.

a, EcN levels in each tissue 50 days after a single oral EcN inoculation administered to 6–8 week old WT adult mice on the C57BL/6 background reared under specific pathogen free laboratory conditions (n = 6). b, anti-EcN IgG end point (EcEPT) titres in the sera of mice 50 days after EcN oral inoculation (n = 8) compared with control mice without E. coli colonization (n = 7) using three independently prepared batches of EcN coated ELISA plates. c, anti-EcN titres for each isotype in the sera of mice 50 days after EcN oral inoculation (n = 7–10 mice, with each dot representing the data from an individual animal) compared with control mice without E. coli colonization (n = 7–10 mice, with each dot representing the data from an individual animal). d, Sequence type, O-antigen, K-capsule and flagella serotype for each E. coli clinical isolate. e, IgG end point (EcEPT) titres against each E. coli strain described in panel d in the sera of adult mice 50 days after a single oral EcN inoculation (n = 8–14 mice/group, with each dot representing the data from an individual animal) compared with no E. coli colonization control mice (n = 5–11 mice/group, with each dot representing the data from an individual animal). Data are presented as mean values (bar) ± one standard deviation. Differences between groups analysed using unpaired Student’s t test. LOD, limits of detection.

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