Fig. 2: Structure and function of the MBV GP glycan cap.
From: Structures of Marburgvirus glycoprotein and its complex with NPC1 receptor
a, Comparison of the RBS-interacting glycan cap loops in RAVV GPcl and EBOV GP-ΔM (bound to a glycan cap-targeting nanobody; Protein Data Bank (PDB) ID: 9BSU). The RAVV RBS is shown as a pink surface, whereas the glycan cap loops from RAVV and EBOV are displayed as cyan and grey ribbons, respectively. N* and C* indicate the termini of the RAVV glycan cap loop. The amino acid sequences of the two loops — loop 207 (RAVV) and loop 221 (EBOV) — are also shown. b, Detailed structural comparison of the glycan cap loops in RAVV GPcl (cyan) and nanobody-bound EBOV GP-ΔM (grey). c–f, SPR analysis. NPC1-C binds to RAVV GPcl (c) but not to RAVV GP-ΔM (d), whereas Nanosota-MB1 binds to RAVV GPcl with ultra-high affinity (e) and to RAVV GP-ΔM with lower, but still strong, affinity (f). For NPC1-C binding to RAVV GPcl, SPR was performed in three independent replicates; mean Kd values from all replicates and a representative binding curve are shown in c (see Supplementary Fig. 2 for all curves). RU, response unit.
