Extended Data Fig. 3: Determinants of branching circuit performance.
a, Branching circuit architecture in which ten constitutive yeast promoters were systematically placed on left and right arms flanking the recombination module, generating 90 designs with distinct Venus-to-mScarlet ratios (mean ± s.d., n = 8 biological replicates). b, Sixteen configurations generated by permuting the orientation of three recombination sites with fixed flanking promoters (pTEF1 left, pTEF2 right) (mean ± s.d., n = 8 biological replicates). c, Performance of circuits containing Bxb1 att sites with orthogonal central dinucleotide variants (GA, GT, TC, TT, CA, CC, CT) in otherwise identical cassettes (mean ± s.d., n = 8 biological replicates). d, Effect of alternative payloads on expression balance, with Venus replaced by other recombinases upstream of pTDH3-driven mScarlet (mean ± s.d., n = 8 biological replicates). e, Comparison of branching circuits catalysed by four serine integrases (Bxb1, A118, R4, TP901) using identical promoter contexts (mean ± s.d., n = 8 biological replicates).
